The plaintiffs argued that because the amendment's language only mentions smoking in public, users should be allowed to smoke in private.
There are eight cases against the state, ranging from smoking to how the state awards licenses for medical marijuana treatment centers.
Two months ago, Roger Stone, a longtime adviser to President Trump, revealed that he was forming a new bipartisan coalition to defend state marijuana laws.
Gov. Rick Scott has signed into law a bill that outlines how patients can qualify and receive medical marijuana. Critics are already planning a lawsuit.
The Florida Legislature's passage of a bill to enact the state's amendment expanding the use of medical marijuana has ended one chapter in the battle.